.six 47.362.4 47.062.1 34.962.six 33.761.eight 33.261.14.561.0 20.361.3 21.862.two 23.061.five 29.062.1 29.461.two 25.661.4.360.1 4.560.2 5.260.1 five.160.two three.660.2 four.560.2 5.160.12.560.1 14.260.7 14.860.two 13.460.5 23.761.two 15.760.six 18.360.75.360.2 74.961.1 74.660.2 73.360.2 59.560.8 72.660.six 70.760.12.260.2 ten.960.five 10.660.2 13.360.six 16.860.four 11.760.two 11.060.8166 5865 5363 5565 3864 3563Mean values are provided in percentage of seed dry weight with typical deviations. n?. doi:ten.1371/journal.pone.0062099.tPLOS 1 | plosone.orgSeed Structure of Ultrahigh Oil Content RapeseedFigure 2. Seed morphology of 7 rapeseed lines with distinctive oil content. The upper is the ultrahigh oil content material line YN171, the middle is three higher oil content material lines ZY036, 6F313 and 09?eight, the nether is 3 low oil content lines 51070, 7ml177-3 and 9Q629-3. Bar is 1 cm. doi:10.1371/journal.pone.0062099.gA considerable negative correlation between the oil and protein contents and also the seed coat ratio existed, with correlation coefficients of 20.74 (p,0.01) and 20.54 (p,0.01), respectively. Around the contrary, a significant good correlation was observed involving the oil content plus the cotyledon ratio, using a correlation coefficient of 0.43 (p,0.01). The seed coat ratio had a important adverse correlation using the cotyledon ratio, along with the coefficient was 20.90 (p,0.01). Even so, there was a significant optimistic correlation in between the seed coat ratio plus the radicle ratio, with a coefficient of 0.35 (p,0.01). The cotyledon ratio had a substantial negative correlation with all the radicle ratio, plus the coefficients was 20.PLOS One | plosone.org(p,0.01). Even so, there was a considerable positive correlation in between the cotyledon ratio plus the 1000-seed weight, with a coefficient of 0.43 (p,0.01). There was a significant unfavorable correlation amongst the radicle ratio and the 1000-seed weight, using a correlation coefficient of 20.60 (p,0.01).Analysis of Correlation amongst Oil Content and Oil Physique Organelle to Seed RatioRapeseed seed is consisted of seed coat, cotyledon and radicle. The oil body organelle is accumulated in aleurone cells of seed coat, cotyledon cells and radicle cells. The oil physique organelle toSeed Structure of Ultrahigh Oil Content material RapeseedFigure three. TEM analysis of 7 lines different oil content material rapeseed mature cotyledons.Prussian blue insoluble web The upper could be the ultrahigh oil content material line YN171 with 3 observations (YN171-1, YN171-2 and YN171-3), the middle is three high oil content material lines ZY036, 6F313 and 09?8, the nether is three low oil content lines 51070, 7ml177-3 and 9Q629-3.425380-37-6 In stock o, oil physique; p, protein physique; Bar is two mm.PMID:23996047 doi:ten.1371/journal.pone.0062099.gcell location ratios for aleurone cells, cotyledon cells, and radicle cells could be measured by TEM analysis (Table 1). The aleurone (about 1/3 of seedcoat), cotyledon and radicle ratios to seed also are measured in Table 1. Therefore the oil body organelle to seed ratio may be calculated. So as to analyze the connection involving oil content with all the oil physique organelle to seed ratio, an expression was utilised to calculate the oil physique organelle to seed ratio as following: the oil body organelle to seed ratio = (Cotyledon ratio 6 Oil physique organelles ratio in cotyledon cell+Radicle ratio six Oil body organelles ratio in radicle cell +1/3 6 Seedcoat ratio 6 Oil body organelles ratio in aleuronic cell). In line with the information in table 1, the oil body organelle to seed ratio of seven rapeseed lines was 67.3, 48.6, 45.eight, 46.3, 29.1, 29.5 and 27.9 respect.